Catalog Number - 85318-05P-2

The 85318‑05P‑2 Cogent RP C18 ABX™ HPLC Column is engineered for robust separations of compounds with extreme pKa values, offering stable performance across a wide pH range (2–12) using a bulky end‑capped C18 bonded phase on high‑purity silica.

This design reduces unwanted interactions and improves peak shape for acidic, basic, and neutral analytes, making it ideal for challenging reversed‑phase applications.

✅ Ideal as a USP L1 alternate for methods involving strong acids or bases

50 mm Length – Fast LC‑MS & Method Scouting

• Designed for rapid separations and short run times

• Ideal for method development and screening workflows

• Reduces:

  • Solvent consumption

  • Analysis cycle times

✅ Preferred for fast LC‑MS method development and high‑throughput labs

2.1 mm ID – LC‑MS / Low‑Flow Applications

• Optimized for low‑flow HPLC and LC‑MS systems

• Enhances MS sensitivity due to reduced solvent flow

Typical Flow Rate Range

• ~0.2–0.3 mL/min

✅ Requires precise low‑flow pump capability and low‑volume detection systems

3 µm Particle – High Efficiency & Practical Performance

• Higher efficiency than 5 µm columns

• Produces:

  • Sharper peaks

  • Improved resolution

• Lower backpressure than 1.8 µm UHPLC columns

✅ Ideal for:

• High‑performance HPLC systems

• LC‑MS methods without UHPLC pressure limits

Wide pH Stability (pH 2–12) – Extreme Method Capability

• Designed for acidic and basic mobile phase conditions

• Suitable for:

  • Strong acids

  • Strong bases

• Maintains performance in aggressive analytical environments

✅ Enables method flexibility beyond conventional C18 phases

Why Choose RP C18 ABX™ Columns (3 µm)

Extreme pH Ruggedness

• Stable across full pH range (2–12)

• Ideal for challenging analytes and harsh conditions

Balanced Efficiency & Pressure

• More efficient than 5 µm columns

• Lower pressure than 1.8 µm UHPLC columns

Improved Peak Shape for Acids & Bases

• Advanced end‑capping reduces secondary interactions

• Enhances chromatographic reproducibility

✅ Excellent choice for method development on standard HPLC systems

LC‑MS & Narrow‑Bore Performance Advantages

• Short, narrow‑bore format:

  • Enables fast analysis and reduced solvent use

  • Enhances sensitivity in MS detection

• Frequently used in:

  • LC‑MS and LC‑MS/MS workflows

✅ Ideal for high‑efficiency, low‑volume analytical systems

System & Sample Considerations

• Designed for HPLC or UHPLC systems capable of low‑flow operation

• Recommended sample preparation:

  • 👉 Use 0.2 µm syringe filters for proper sample preparation before injection

✅ Helps:

• Prevent column blockage

• Maintain performance and longevity

Helpful Resources & Links

Typical Applications

• LC‑MS and LC‑MS/MS analysis

• Acidic and basic compound separations

• Pharmaceutical method development

• Stability‑indicating methods

• Long‑chain fatty acids

• Complex mixture separations under extreme pH

Important Usage Notes

• Designed for low‑flow analytical and LC‑MS systems (2.1 mm ID)

• Requires precise pump delivery at low flow rates (~0.2–0.3 mL/min)

• Suitable for extreme pH mobile phase conditions (pH 2–12)

• Monitor backpressure to ensure system compatibility